Fiehn Laboratory for Untargeted Metabolomics

The Fiehn research laboratory develops improved methods in analytical chemistry and bioinformatics to capture and utilize metabolomic data. These tools are employed to understand, which parts of larger biochemical networks respond to genetic perturbation or environmental stress.

Untargeted metabolomics by LC-accurate mass MS/MS analysis

Using targeted assays, e.g. by LC-QTRAP mass spectrometers, yields high confidence data on a range of metabolites from different biochemical pathways. However, this view assumes we already know everything there is to know about cellular and physiological metabolism! This assumption is certainly false. We routinely detect hundreds of novel metabolic signals in liquid chromatography-mass spectrometry profiling. Unlike gas chromatography, such LC-based methods are much more selective in terms of the compounds that can be seen in any specific method. For example, our lipidomic assays (see Core Services) have been implemented as routine tools for blood plasma or blood serum analysis, detecting over 600 identified complex lipids from a range of different lipid classes. However, such method is blind for hydrophilic compounds, including compounds that are critical in cardiovascular diseases (e.g. carnitine, creatinine, choline, betaine), gut microbial metabolites (such as sulfates) or polysaccharides and glucuronidated metabolites. Many transformations are carried out by mammalian endogenous enzymes due to substrate (or reaction) unspecificity, and, equally important, many biochemical reactions are performed by microbes living within the human super-organism. In addition, humans eat plants.... All such biochemicals can therefore be found as 'unknowns' in LC-MS or GC-MS profiling scans, and these compounds are certainly not yet covered by databases like HMDB or KEGG.

We therefore engage with interested biological or clinical scientists to develop LC-methods, including sample preparation, to better address this chemical complexity. We use a range of different columns, including hydrophilic interaction chromatography, to look for polar compounds; we devise novel ways to acquire and process such complex MS and MS/MS data, and we test different mass spectrometers in conjunction with instrument vendors. These projects should lead to collaborative research papers and NIH grant submissions.

If interested, please contact Professor This email address is being protected from spambots. You need JavaScript enabled to view it. (This email address is being protected from spambots. You need JavaScript enabled to view it.) for more information, and visit the Fiehn research website.

Fiehn research and WCMC service core lab:

10 LCMS instruments

2x Agilent 6530 QTOF mass spectrometer 
1x Agilent 6550 QTOF mass spectrometer 
1x Sciex QTRAP 6500 mass spectrometer
1x Sciex Triple TOF mass spectrometer 
2x ThermoFisher QExactive MS mass spectrometers
1x ThermoFisher ion trap LTQ mass spectrometer
 
2x ThermoFisher Altis triple quadrupole mass spectrometers

8 GCMS instruments

1x Agilent GC-QTOF mass spectrometer for chemical or electron ionization
2x Agilent GC-quadrupole mass spectrometer with autosampler and s/sl injector or APEX large volume injector
5x LECO Pegasus III, IV and BT GC-time of flightmass spectrometers

Staff

645f4c76 b61a 457c 9010 100c6380ae8f

This photo (Jan 2015) shows Fiehn research and metabolomics core service lab members.
Photo: Jeannette Martins

Director: Oliver Fiehn, PhD, Professor (Molecular and Cellular Biology, Genome Center, College of Biological Sciences at UC Davis)

Current Fiehn research lab members

Current Fiehn research lab projects

Current Fiehn research papers